A simple method for chromosome banding.

Karyotype Analysis and Chromosome Banding

Nearly all methods of chromosome banding rely on harvesting chromosomes in mitosis. This is usually achieved by treating cells with tubulin inhibitors, such as colchicine or demecolcine (colcemid), that depolymerize the mitotic spindle and so arrest the cell at this stage. Excessively long incubations with Colcemid result in overcondensed chromosomes that band poorly and moreover some cell type...

A convenient Simple Method for Synthesis of Meta-iodobenzylguanidine (MIBG)

Radioiodinated meta iodobenzylguanidine (MIBG) is one of the important radiopharmaceuticals in Nuclear Medicine. [123/131I]MIBG is used for imaging of Adrenal medulla, studying heart sympathetic nerves, treatment of pheochromacytoma and neuroblastoma. For clinical application, radioiodinated MIBG is prepared through isotopic exchange method, which includes replacement of radioactive iodine in a...

A new chromosome banding technique, spectral color banding (SCAN), for full characterization of chromosomal abnormalities.

We have developed spectral color banding (SCAN) as a new chromosome banding technique based on spectral analysis of differentially labeled chromosome band-specific painting probes. In this study, we succeeded in displaying a multicolor banding pattern for chromosome 3, which was almost identical to the pattern obtained with the corresponding G-banding. We applied this method to metaphase cells ...

A Simple Method for Modeling Open Cracked Beam

Abstract A simple method is proposed to model the open cracked beam structures. In this method, crack is modeled as a beam element. Hence cracked beam can be assumed to be a beam with stepped cross sections, and problem of determining natural frequency and mode shape of cracked beam, can be solved as determining these characteristics for a beam with different length and cross section. With this...

A simple method for the determination of serum cholesterol